Application

Research Sub CategoryFragment Specific Secondary Antibodies

Western Blotting: 1:5,000 -1:50,000
ELISA: 1:5,000 -1:50,000

Optimal working dilutions must be determined by the end user.

Research CategorySecondary & Control Antibodies

This Mouse anti-Rabbit light chain Antibody, Alkaline Phosphatase conjugate is validated for use in ELISA, WB for the detection of Rabbit light chain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Immunogen

Epitope: Kappa light chain

Prepared from rabbit IgG light chain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Ascites

Isolated from ascites by chromatographic procedures. Liquid in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 with 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.

Specificity

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Target description

25 kDa